Priya, Kannappan and Janani, Balakarthikeyan (2020) International Journal of Biological Macromolecules.

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Abstract

In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was
synthesized using shrimp shell extracted chitin as reducing, capping and stabilizing factor under
the optimized conditions. Also, the anticancer potential of synthesized biogenic AgNPs was
evaluated against human hepatocarcinoma (HepG2) cells. Ultraviolet visible spectroscopy (UVVis spec) study indicated that the development of AgNPs present in the colloidal solution was
single peak at 446 nm. FTIR results showed a strong chemical interaction between the chitin and
biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites. The SEM
TEM analytical studies confirmed the synthesized AgNPs had a spherical shape crystalline
structure with size ranges from 17 to 49 nm; EDX study also confirmed the percentage of weight
and atomic elements available in the colloidal mixture. Furthermore, the synthesized AgNPs
showed significant cytotoxic effect on the HepG2 cells with an IC50 value shown at 57 ± 1.5
µg/ml. The apoptotic and necrotic cell death effects of AgNPs were also confirmed by flow
cytometry. The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9,
PARP and downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells,
confirmed the anticancer potential of AgNPs. These findings suggest that the AgNPs possess
significant anticancer activity against HepG2 cells which could play major role in the therapeutic drug development to treat cancer in future.

Item Type: Article
Uncontrolled Keywords: chitin, AgNPs, TEM, HepG2, HCC, cytotoxicity, apoptosis
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 14 Mar 2023 07:30
Last Modified: 14 Mar 2023 07:30
URI: http://ir.psgcas.ac.in/id/eprint/1769

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