Suseela, Vivekananthan and Muthukrishnan, Pallikondaperumal and Shanmuga Priya, Ramasamy (2022) Eco-Friendly Preparation of Silver Nanoparticles and Their Antiproliferative and Apoptosis-Inducing Ability against Lung Cancer. Life. pp. 1-19.

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Abstract

In the present study, the anti-proliferative and apoptotic potential of Tabebuia roseo-alba
in lung cancer was assessed. Silver nanoparticles (AgNPs) of T. roseo-alba were synthesized using
an ethanolic extract and characterized by adopting various parameters. Herein, the eco-friendly,
cost-effective, and green synthesis of AgNPs was evaluated using an ethanolic extract of T. roseo-alba.
The as-synthesized AgNPs were then characterized using various characterization techniques, such
as UV-visible spectroscopy (UV-vis), X-ray powder diffraction (XRD), dynamic light scattering (DLS),
scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The AgNPs
are crystalline, spherical, and highly stable AgNPs of varying sizes in the range of 5–20 nm. The
anticancer activity of the ethanolic extract of T. roseo-alba and its AgNPs was determined using an
MTT assay. The results indicated that, although both samples showed prominent anti-proliferative
activity on lung cancer cell lines, the AgNPs of T. roseo-alba were found to be more potent than the
ethanolic extract. Further, apoptosis induction ability was evaluated by FITC Annexin V and PI
staining, the results of which demonstrated the efficiency of the ethanolic extract of T. roseo-alba and
its AgNPs in causing oxidative stress and subsequent cellular death. This was subsequently further
confirmed by measuring the mitochondrial membrane potential after staining the cells with JC1. The
apoptotic mode of cell death was further confirmed by DNA fragmentation and caspase assays using
Western blot analysis.

Item Type: Article
Uncontrolled Keywords: anti-proliferative; apoptosis; green synthesis; silver nanoparticles; lung cancer
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 23 Mar 2023 06:45
Last Modified: 23 Mar 2023 06:45
URI: http://ir.psgcas.ac.in/id/eprint/1816

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