Michael, A and Naveen, S (2019) Isolation and identification of enterotoxigenic Staphylococcus aureus isolates from Indian food samples: evaluation of in-house developed aptamer linked sandwich ELISA (ALISA) method. J Food Sci Technol, 56 (2). pp. 1016-1026.

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Staphylococcus aureus is one of the major food
contaminants worldwide, and its enterotoxins are documented as food poisoning and bioterrorism agents. In the
present study, an attempt was made to account on the
incidences of toxigenic S. aureus and its antibiotic resistance profiles in ready to eat bakery food products from
different parts of Southern India (Andhra Pradesh, Karnataka, Kerala, Tamil Nadu, and Telangana). A total of 100
food samples, including milk, cake, cheese and chicken
products were assessed for S. aureus and Staphylococcal
Enterotoxin B (SEB) by PCR. Among the subjected food
samples, a total of 51 isolates belong to genus Staphylococcus and out of that, 34 isolates were S. aureus. Among
34 S. aureus isolates, 14 isolates were found positive for
SEB. The PCR results were further co-evaluated with inhouse developed aptamer linked immunosorbent assay
(ALISA) for the specific and sensitive detection of SEB.
The obtained ALISA results were promising and found
consistent with PCR analysis. Furthermore, 24%, 47%,
91%, 82%, 59%, and 47% of S. aureus isolates were found
resistant to chloramphenicol, methicillin, penicillin, ampicillin, erythromycin, and oxacillin, respectively and
concluded as a multidrug resistance (MDR). In conclusion,
the present study revealed high presence of toxigenic and
MDR resistant S. aureus species among the studied regions
of Southern India. The present study cautions the need of
stringent food safety regulations in India to control the
toxigenic and MDR S. aureus from food sources and to
minimize the risks associated with S. aureus

Item Type: Article
Uncontrolled Keywords: Ready to eat bakery foods Staphylococcus aureus Staphylococcal enterotoxin B Polymerase chain reaction Multidrug resistance Aptamer linked immunosorbent assay
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 12 Jul 2022 08:00
Last Modified: 12 Jul 2022 08:00
URI: http://ir.psgcas.ac.in/id/eprint/1300

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