Anitha, P and Balasubramanian Murugesan (2025) Extraction of biologically active protein from ayapana (Ayapana triplinervis Vahl) and evaluation of antimicrobial activity. Extraction of biologically active protein from ayapana (Ayapana triplinervis Vahl) and evaluation of antimicrobial activity, 13 (3). pp. 25-31. ISSN 2320-3862

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Abstract

Peptide therapy is an emerging technology in medicine. Plant-based peptide drug discovery would be an
effective and target-specific therapeutic agent which overcomes the traditional drawback. The study aims
to explore and characterise the potent protein candidate from fresh leaves of Ayapana triplinervis Vahl, to
study the efficiency against various clinical pathogens. The proteins have been extracted, precipitated,
and characterised. Three methods have been used to extract proteins, and the EB TA (Extraction buffer -
TCA acetone) method favours a good yield of protein. The extracted protein was completely solubilised
in Tris-Cl (pH 7.4) buffer than other solubilizing buffers. The molecular weight of the extracted proteins
was determined by plotting a graph of log Molecular weight with relative migration distance for
standards. The proteins separated were less than 30 kDa by all three methods. The proteins extracted by
using the EB-TA method elicited antimicrobial activity against E. coli, Staphylococcus aureus,
Streptococcus species and MRSA (Methicillin-Resistant Staphylococcus aureus) strains isolated from
clinical wounds. The above results suggest that the protein extract of the fresh leaves of Ayapana
triplinerve Vahl has potent antibacterial activity

Item Type: Article
Uncontrolled Keywords: Ayapana triplinerve Vahl, protein, peptides, log Molecular weight, antibacterial activity, solubilization
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Dr. B Sivakumar
Date Deposited: 01 Apr 2026 07:18
Last Modified: 01 Apr 2026 07:18
URI: https://ir.psgcas.ac.in/id/eprint/2766

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