Rajiv, P (2025) Green Synthesis and Characterization of SiO2 Nanoparticles Using Tridax Procumbens Leaf Extract and Enhancing the Invitro Wound Healing Activity in L929 Fibroblast Cell Lines. Green Synthesis and Characterization of SiO2 Nanoparticles Using Tridax Procumbens Leaf Extract and Enhancing the Invitro Wound Healing Activity in L929 Fibroblast Cell Lines.

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Abstract

This study aims to synthesize silica nanoparticles (SiO2-NPs) using Tridax procumbens leaves. Previous studies have
demonstrated the effectiveness of the leaf juice of Tridax procumbens in treating and healing fresh wounds. SiO2-NPs
have been found to enhance topical wound healing by promoting the migration of skin fibroblasts. Therefore, the current
research focused on the green synthesis of SiO2-NPs from Tridax procumbens leaves extract and evaluating their scratch
wound healing activities. The synthesized SiO2-NPs were characterized using various techniques like XRD, UV-visible
spectrophotometry, FTIR, FESEM, EDX, Zeta potential and TG analysis. The characterization analysis confirmed that
the particles were spherical, containing silicon (Si) and oxygen (O), exhibiting an average size of 44 nm, and experienc�ing a mass loss of 48.9%. The antioxidant activity of the synthesized SiO2-NPs was evaluated through the DPPH assay,
revealing an IC50 value of 48.8 µg/mL. This indicates their capability to diminish free radical generation and alleviate
oxidative stress. Moreover, the study aimed to explore the efficacy of these nanoparticles in wound healing activity. The
in vitro scratch assay was employed to assess wound closure of L929 fibroblast cell lines. The results revealed that the
synthesized SiO2-NPs showed higher wound healing activity (74%) in L929 fibroblast cell lines than the control at 24 h,
indicating the ability of the synthesized nanoparticles in enhancing wound closure processes. Overall, this study analyses
the potential of plant-mediated synthesis of SiO2-NPs for biomedical applications, particularly in wound healing therapies.

Item Type: Article
Uncontrolled Keywords: Tridax procumbens · SiO2-NPs · XRD · EDX · Scratch wound healing · (DPPH) assay
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Dr. B Sivakumar
Date Deposited: 07 Apr 2026 11:37
Last Modified: 07 Apr 2026 11:37
URI: https://ir.psgcas.ac.in/id/eprint/2781

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