Senthil Kumar, J (2025) Production, partial purification and characterization of amylase from Paenibacillus lactis PEL6, an endophytic bacteria from Mitrephora heyneana. Production, partial purification and characterization of amylase from Paenibacillus lactis PEL6, an endophytic bacteria from Mitrephora heyneana, 20 (8). pp. 101-107.
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Abstract
In recent years, researchers are keenly focusing on
discovering antifungals, enzymes, immune
suppressants and anticancer compounds produced by
endophytic microorganisms, aiming to improve human
well-being. The objective of this study is to produce,
purify and characterize amylase produced by
Paenibacillus lactis PEL6, an endophyte isolated from
Mitrephora heyneana. The enzyme screening study in
starch agar plate demonstrated the activity of amylase
produced by Paenibacillus lactis. In ammonium
sulphate precipitation study, the optimal saturation
level for precipitating amylase was determined as 40-
50%. Ammonium sulphate fractionation increased
specific activity of amylase in crude enzyme from 0.347
to 0.556 and increased the purity of amylase to 1.6-
fold.
The study on the effect of pH and temperature on
amylase revealed that the optimum pH and
temperature for the action of amylase on starch are pH
6.5 and 22°C respectively. The combination of amylase
with commercial detergent was more effective in
removing stains from fabrics than using the
commercial detergent alone. In summary, this study
validates that Paenibacillus lactis can act as a good
source of amylase enzyme, which has great potential to
be utilized in detergent industries.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Enzymes, Amylase, Endophytes, Detergent, Stain removal, Paenibacillus lactis. |
| Divisions: | PSG College of Arts and Science > Department of Biotechnology |
| Depositing User: | Dr. B Sivakumar |
| Date Deposited: | 10 Apr 2026 06:22 |
| Last Modified: | 10 Apr 2026 06:47 |
| URI: | https://ir.psgcas.ac.in/id/eprint/2787 |
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