Balasubramanian Murugesan and Anitha, P (2025) Evaluation of the anti-inflammatory and wound healing potential of Ayapana protein extracted from fresh leaves of Ayapana triplinervis Vahl - In vitro. Next Research (2): 100912. pp. 1-10. ISSN 3050-4759

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Abstract

Peptide-based therapeutics represent promising candidates in modern drug discovery due to their pleiotropic and
multipotent activities. Notably, plant-derived proteins and peptides provide an untapped source of novel phar
macological agents, with plant peptides constituting a rich and underexplored reservoir of bioactive molecules.
In this study, we delineate the wound-healing and chronic wound healing mediated anti-inflammatory efficacy of
the Ayapana protein, isolated from the fresh leaves of Ayapana triplinervis Vahl. Ayapana protein inhibited heat-
induced erythrocyte lysis by 70 % at 2.5 µg/ml in a membrane stabilisation assay and suppressed albumin
denaturation with an IC50 value of 298.42 µg/ml. Moreover, it scavenged nitric oxide radicals generated by
sodium nitroprusside. In RAW 264.7 macrophages, the proteins significantly down regulated proinflammatory
mediators COX-2, IL-6, TNF
α
, and Leukotriene B4. In a scratch wound healing assay, Ayapana protein promoted
99 % fibroblast (L929) cell migration within 48 h, without inducing cytotoxic effects. Together, these results
highlight the dual activity (anti-inflammatory and wound healing) of Ayapana protein and underscore its
promise as a novel therapeutic candidate for tissue repair and inflammation-mediated chronic wound repair.

Item Type: Article
Uncontrolled Keywords: Albumin denaturation Cell proliferation Anti-inflammatory Wound healing activity Proinflammatory cytokines
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Dr. B Sivakumar
Date Deposited: 19 Jun 2026 06:20
Last Modified: 19 Jun 2026 06:20
URI: https://ir.psgcas.ac.in/id/eprint/2937

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