Victor Arokia Doss, D and Abitha Devi, N (2004) Evaluation on the Use of β-Lactamase and Aminoglycoside modifying enzyme gene sequences as markers for the early detection of antibiotic resistance profile of Pseudomonoas aeruginosa. Evaluation on the Use of β-Lactamase and Aminoglycoside modifying enzyme gene sequences. ISSN 0278-0240

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Abstract

Pseudomonas aeruginosa is one of the major causes of infections including the hospital acquired (Nosocomial)
infections. Detection of them and their antibiotic resistance profile by conventional method takes about three days. Recently,
DNA based diagnostic methods are being used for the identification of the pathogens. Hence we have tested a rapid and sensitive
method using DNA sequences as markers for detecting the presence of three genes coding for the enzymes that inactivate
the two most commonly used Anti – pseudomonadal drugs such as β-lactam antibiotics (Penicillin, and its derivatives) and
Aminoglycosides such as Gentamicin, Tobramycin, Amikacin, Streptomycin. The internal region of these genes were used for
designing and synthesizing primers and these primers were used in Polymerase Chain Reaction (PCR) to screen for the presence
of these genes in the clinical isolates and to label them non-radioactively with Biotin. They in turn were used to detect the presence
of the antibiotic resistance genes in the clinical isolates by hybridization. The specificity (ratio of positive results obtained in both
methods and the sensitivity (the minimum amount of sample DNA and the labeled probe required for the tests) were evaluated.

Item Type: Article
Uncontrolled Keywords: Gene marker, DNA probes, antibiotic resistance, drug-modifying enzymes, non-radioactive labelling, anti-biogram, culture and sensitivity test
Depositing User: Mr Team Mosys
Date Deposited: 12 Aug 2022 04:28
Last Modified: 12 Aug 2022 04:28
URI: http://ir.psgcas.ac.in/id/eprint/1448

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