Sakthivel, K M (2021) Targeting NF-kB mediated cell signaling pathway and inflammatory mediators by 1,2-diazole in A549 cells in vitro. Biotechnology Reports, 21. pp. 1-9. ISSN 215-017X

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Abstract

Lung cancer is the leading cause of cancer deaths globally. The objective of this study was to investigate
the effect of 1,2-diazole (pyrazole) as an anti-cancer drug on human non-small cell lung carcinoma A549
cells. We attempt to examine the expression level of pro-inflammatory proteins such as TNF-α, NF-kBp65, MMP-2 and E-Cadherin which are commonly associated with an inflammatory response in epithelial
cells and apoptosis in A549 cells. The LPS-induced cytokines and inflammatory mediators include TNF-α,
IL-6, iNOS and COX-2 levels in A549 cells and the effect of pyrazole was studied. The present study reveals
that, pyrazole inhibits A549 cells by suppressing TNF-α induced MMP-2 expression, thereby inhibiting
the nuclear translocation of NF-kB-p65. Pyrazole significantly up-regulate the E-cadherin level and
down-regulated MMP-2 expression that could probably preventing A549 cancer cells to invade. The
study further substantiated the anti-cancer property of pyrazole by regulating the above mentioned level
of LPS-induced cytokines and inflammatory mediators. The observations of the present study open a
possibility for the development of an effective therapeutic agent that targets inflammatory and signaling
pathway mediators to challenge human non-small cell lung carcinoma

Item Type: Article
Uncontrolled Keywords: 1,2-diazole Cell signaling Pyrazole A549 cells Inflammatory mediators Lung cance
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 11 Jun 2022 06:25
Last Modified: 11 Jun 2022 06:25
URI: http://ir.psgcas.ac.in/id/eprint/1216

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