Brindha, Durairaj and Sobanalakshmi, Srinivasan (2021) Collagen isolation and characterization from Sardinella longiceps. JOURNAL OF ADVANCED VETERINARY AND ANIMAL RESEARCH, 8 (4). pp. 679-686. ISSN 2311-7710

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Abstract

Objective: Collagen is a fibrous protein that is primarily used in the pharmaceutical and biomedical industries. Thisstudy isolates and characterizestype-1 collagen from Sardine longiceps(scales,
skin, and muscle).
Materials and Methods: Collagen was isolated from S. longiceps using two methods: acid-solubilized collagen and pepsin-solubilized collagen. Sodium dodecyl sulfate–polyacrylamide gel
electrophoresis (SDS–PAGE) was used to estimate the molecular weight of isolated collagen.
Ultraviolet (UV)-visible spectrophotometry analysis was used to confirm the collagen extracted
(type-I collagen). The functional groups of isolated collagens were identified using fourier transform infrared (FTIR) analysis. The X-ray diffraction (XRD) technique was used to investigate the
crystallinity of isolated collagen. The high-pressure liquid chromatography (HPLC) technique was
used to study the amino acid composition.
Results: SDS–PAGE of S. longiceps revealed molecular weights ranging from 116 kDa for α-2 to
97 kDa for α-1. UV-visible spectra showed an absorbance value below 300 nm, and the results
confirmed type-I collagen. FTIR showed major functional groups like amide A, B, I, II, and III. XRD
determined the crystallinity of isolated collagen. The HPLC results showed the presence of higher
glycine content, followed by proline and hydroxyl proline in the extracted collagen.
Conclusion: The overallstudy confirmed that fish w

Item Type: Article
Uncontrolled Keywords: Isolation of fish collagen; Sardinella longiceps; acid-soluble collagen; pepsin-soluble collagen characterization
Depositing User: Mr Team Mosys
Date Deposited: 13 Jun 2022 03:05
Last Modified: 13 Jun 2022 03:05
URI: http://ir.psgcas.ac.in/id/eprint/1219

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