Shobana, CS (2019) Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of Fusarium and Aspergillus Isolates from Corneal Scrapings. Hindawi BioMed Research International: 6395840. pp. 1-9.

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Abstract

Fungal aetiology of keratitis/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in
developing countries including India. More importantly, Fusarium and Aspergillus are reported commonly implicating corneal
ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the
two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis
patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies, Fusarium (216,
52.5% of 411) andAspergillus(68, 16.5% of 411) were predominantly determined.While the study revealed a male preponderance with
both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active,
respectively, against Fusarium spp. and Aspergillus spp. Additionally, 94.1% of culture proven Fusarium keratitis and, respectively,
100% and 63.6% of A. favus and A. fumigatus were confrmed by multiplex PCR. Te sensitivity of the PCR employed in the present
study was noted to be 10 fg/�l, 1 pg/�l, and 300 pg/�l of DNA, respectively, for Fusarium, A. favus, and A. fumigatus. Alarming fact
was that Fusarium and Aspergillus regionally remained to be the common cause of mycotic keratitis and the Fusarium isolates had
a higher antifungal resistance than Aspergillus strains against most of the test drugs.

Item Type: Article
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 14 Jul 2022 06:21
Last Modified: 14 Jul 2022 06:21
URI: http://ir.psgcas.ac.in/id/eprint/1306

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