Priya, K and Janani, B (2020) Shrimp shells extracted chitin in silver nanoparticle synthesis: Expanding its prophecy towards anticancer activity in human hepatocellular carcinoma HepG2 cells. International Journal of Biological Macromolecules. pp. 1402-1409. ISSN 0141-8130

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Abstract

In this study, a well-organized, simplistic, and biological route of AgNPs (AgNPs) was synthesized using shrimp
shell extracted chitin as reducing, capping and stabilizing factor under the optimized conditions. Also, the anticancer potential of synthesized biogenic AgNPs was evaluated against human hepatocarcinoma (HepG2) cells.
Ultraviolet visible spectroscopy (UV–Vis spec) study indicated that the development of AgNPs present in the colloidal solution was single peak at 446 nm. FTIR results showed a strong chemical interaction between the chitin
and biogenic AgNPs; whereas, XRD studies confirmed AgNPs presence in the composites. The SEM TEM analytical
studies confirmed the synthesized AgNPs had a spherical shape crystalline structure with size ranges from 17 to
49 nm; EDX study also confirmed the percentage of weight and atomic elements available in the colloidal mixture. Furthermore, the synthesized AgNPs showed significant cytotoxic effect on the HepG2 cells with an IC50
value shown at 57 ± 1.5 μg/ml. The apoptotic and necrotic cell death effects of AgNPs were also confirmed by
flow cytometry. The upregulated apoptotic related proteins Bax, cytochrome-c, caspase-3, caspase-9, PARP and
downregulated anti-apoptotic related proteins Bcl-2 and Bcl-xl in cancer cells, confirmed the anticancer potential
of AgNPs. These findings suggest that the AgNPs possess significant anticancer activity against HepG2 cells which
could play major role in the therapeutic drug development to treat cancer in future.

Item Type: Article
Uncontrolled Keywords: Chitin AgNPs TEM HepG2 HCC Cytotoxicity Apoptosis
Depositing User: Mr Team Mosys
Date Deposited: 26 Jul 2022 09:08
Last Modified: 26 Jul 2022 09:08
URI: http://ir.psgcas.ac.in/id/eprint/1363

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