Priya, K and Janani, B (2020) Chitosan-mediated synthesis of biogenic silver nanoparticles (AgNPs), nanoparticle characterisation and in vitro assessment of anticancer activity in human hepatocellular carcinoma HepG2 cells. International Journal of Biological Macromolecules. pp. 844-852. ISSN 0141-8130

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Abstract

The biopolymer chitosan is currently in widespread use because of its nontoxicity, biocompatibility and biodegradability. Therefore, in this study, chitosan extracted from shrimp shells was used to synthesise biogenic silver
nanoparticles (AgNPs). UV–visible spectrophotometry of reduced silver nanoparticles in the colloidal solution
showed a single peak at 400 nm, confirming the formation of AgNPs. The presence of biomolecules responsible
for reducing and capping the biogenic AgNPs was confirmed by FTIR. Surface morphology of the biosynthesised
AgNPs was characterised using SEM, and TEM analysis showed the formation of spherical shapes 17–50 nm. The
presence of elemental silver in the synthesised biogenic AgNPs was confirmed using EDX and the crystalline
structure characterised by XRD. Cytotoxicity of biogenic AgNPs was determined using MTT and the trypan blue
exclusion assay. Morphological changes in HepG2 cells were detected by analysis of the DNA ladder pattern via
gel electrophoresis, and the IC50 of HepG2 cell inhibition by AgNPs was 48 μg/ml. The upregulated caspase 3
and 9 protein expression results confirmed cell death via apoptosis. In conclusion, chitosan has the ability to synthesise AgNPs with in vitro apoptotic activities.

Item Type: Article
Uncontrolled Keywords: Chitosan Nanoparticle TEM Cytotoxicity Apoptosis
Depositing User: Mr Team Mosys
Date Deposited: 03 Aug 2022 08:42
Last Modified: 03 Aug 2022 08:42
URI: http://ir.psgcas.ac.in/id/eprint/1406

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