Hemalatha, D (2020) Pyriproxyfen induced impairment of reproductive endocrine hom e ostasis and gonadal histopathology in zebrafish (Danio rerio) by altered expression of hypothalamus - pituitary -gonadal (HPG) axis genes. Science of the Total Environment, 735. pp. 1-36.

[thumbnail of 10.1016@j.scitotenv.2020.139496.pdf] Text
[email protected] - Published Version
Download (2MB)

Abstract

Pyriproxyfen (PPF
), a broad
-spectrum of insecticide known to cause reproductive and
endocrine disruption in invertebrate
s, while the data is scarce in aquatic vertebrate
s. The goal
of this study is to investigate the impact of PPF on reproductive endocrine system of male
and female zebrafish along hypothalamus
-pituitary
-gonadal (HPG) axis. In brain, PPF caused
significant alteration in the transcript
s of erα
, lhβ, and cyp19b genes in male and fshβ, lhβ,
and cyp19b genes in female zebrafish
. The downstream genes of steroidogenic pathway like
,
star, 3βhsd, 17βhsd, and cyp19a expression were significantly altere
d in gonad of both sexes.
Subsequent changes in circulatory steroid hormone level
s lead to imbalance in hormone
homeostasis as revealed from estradiol/testosterone (E
2/T) ratio. Further, the vitellogenin transcript level was
enhanced in hepatic tissues and their blood plasma content was increased
in male (16.21%) and declined in female (21.69%)
. PPF also induced histopathological
changes in gonads such as
, reduction of mature spermatozoa in male and vitellogenic oocytes
in female zebrafish. The altered
E
2/T ratio and gonadal histopatholog
y
were supported by the
altered transcript levels of HPG axis genes. Overall, these findings provide new insights of
PPF in zebrafish reproductive system and highlights for further investigations on its potential risks in aquatic environment.

Item Type: Article
Uncontrolled Keywords: Endocrine disruption, Testosterone, Estradiol, Vitellogenin, Testes, Ovary
Divisions: PSG College of Arts and Science > Department of Zoology
Depositing User: Mr Team Mosys
Date Deposited: 16 Aug 2022 05:03
Last Modified: 16 Aug 2022 05:03
URI: http://ir.psgcas.ac.in/id/eprint/1462

Actions (login required)

View Item
View Item
Altmetric