Sathyabhama, M (2022) Diferential host responses of sugarcane to Colletotrichum falcatum reveal activation of probable efector triggered immunity (ETI) in defence responses. Plant Cell Reports, 41. pp. 1461-1476.

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Abstract

The complex polyploidy of sugarcane genome and genetic variations in diferent cultivars of sugarcane remain
a challenge to identify and characterise specifc genes controlling the compatible and incompatible interactions between
sugarcane and the red rot pathogen, Colletotrichum falcatum. To avoid host background variation in the interaction study,
suppression subtractive hybridization (SSH)-based next-generation sequencing (NGS) technology was used in a sugarcane
cultivar Co 7805 which is compatible with one C. falcatum pathotype but incompatible with another one. In the incompatible interaction (ICI—less virulent) 10,038 contigs were assembled from ~ 54,699,263 raw reads, while 4022 contigs were
assembled from ~ 52,509,239 in the compatible interaction (CI—virulent). The transcripts homologous to CEBiP receptor
and those involved in the signalling pathways of ROS, Ca2+, BR, and ABA were expressed in both interaction responses. In
contrast, MAPK, ET, PI signalling pathways and JA amino conjugation related transcripts were found only in ICI. In temporal gene expression assays, 16 transcripts showed their highest induction in ICI than CI. Further, more than 17 transcripts
specifc to the pathogen were found only in CI, indicating that the pathogen colonizes the host tissue whereas it failed to do so
in ICI. Overall, this study has identifed for the frst time that a probable PAMP triggered immunity (PTI) in both responses,
while a more efcient efector triggered immunity (ETI) was found only in ICI. Moreover, pathogen proliferation could be
predicted in CI based on transcript expression, which were homologous to Glomerella graminicola, the nearest clade to the
perfect stage of C. falcatum (G. tucumanensis).

Item Type: Article
Uncontrolled Keywords: Sugarcane · Colletotrichum falcatum · Diferential host response · Illumina high throughput sequencing · Defense signalling
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 17 Aug 2022 05:09
Last Modified: 17 Aug 2022 05:09
URI: http://ir.psgcas.ac.in/id/eprint/1476

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