Santhi, Ramalingam (2020) Preliminary evaluation of In vitro and In vivo antioxidative and antitumor activities of flavonoid extract of Tabernaemontana divaricata leaves in Ehrlich’s lymphoma and Dalton’s lymphoma ascites model. 8 Journal of Cancer Research and Therapeutics, 16 (1). pp. 78-87.

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Abstract

Aims: In the present study, the flavonoid fraction of Tabernaemontana divaricata flavonoid fraction (TdFf) leaves was investigated
for its in vitro and in vivo antioxidative and antitumor activity.
Subjects and Methods: The flavonoid fraction of ethyl acetate extract was assessed for their in vitro antioxidant activity by
2,2‑diphenyl‑1‑picrylhydrazyl (DPPH), 2,2’‑azino‑bis (3‑ethylbenzothiazoline‑6‑sulphonic acid) (ABTS), superoxide radicals,
ferric reducing antioxidant power (FRAP), hydrogen peroxide, hydroxyl radicals and nitric oxide and in vivo antioxidative activity by
enzymic and nonenzymic antioxidants in the liver of intraperitoneally implanted Ehrlich’s lymphoma (EAC) and Dalton’s lymphoma
ascites (DLAs) model. The in vitro cytotoxicity was assessed using trypan blue exclusion assay and in vivo antitumor activity was
assessed by screening the ILS, serum liver marker enzymes and histopathology of the liver.
Statistical Analysis Used: The data were expressed as the mean ± standard deviation of the means, and statistical analysis was
carried out employing one‑way and two‑way analysis of variance using Web Agri Stat Package 2.0.
Results: The dose‑dependent percentage scavenging of ABTS, DPPH, FRAP, OH, superoxide radical, and nonradical NO and H2
O2
by TdFf indicated their antioxidative potential. Incubation of EAC/DLA tumor cells with TdFf showed a concentration‑dependent
cytotoxic effect, and the extract killed 50% of EAC/DLA tumor cells at a concentration of 80 µg of TdFf. Coadministration of TdFf
with EAC/DLA‑induced mice showed a significant increase in the liver enzymic and nonenzymic antioxidants and significant decrease
in the serum liver marker enzymes to prove the in vivo antioxidative and antitumor activity of TdFf. It was also confirmed by the
histopathology of the liver.
Conclusions: It may be concluded that the flavonoid fractions of Td possess considerable antioxidative and antitumorigenic activity
against the tested DLA/EAC in both in vitro and in vivo system.

Item Type: Article
Uncontrolled Keywords: Dalton’s lymphoma ascites, Ehrlich’s lymphoma ascites, flavonoid, Tabernaemontana divaricata
Depositing User: Mr Team Mosys
Date Deposited: 17 Aug 2022 08:59
Last Modified: 17 Aug 2022 08:59
URI: http://ir.psgcas.ac.in/id/eprint/1482

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