MANIKANDAN, J and RAFFIEA BASERI, J (2022) Green Synthesized Fe3O4 Nanoparticles as Silymarin Drug Carrier and their Anticancer Activity against Liver-HepG2 and Lung-A549 Cancer Cells. Asian Journal of Chemistry, 34 (9). pp. 2363-2372. ISSN 2363-2372

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Abstract

Iron oxide nanoparticles (IONPs) were synthesized by co-precipitation method with Commiphora berryi latex as stabilizing agent. The
IONPS were coated with PEG2K and then loaded with silymarin drug. The synthesized IONPs, PEG2K and silymarin loaded nanoparticles
were analyzed by FTIR, XRD, SEM and TEM techniques. The FTIR results indicated the coating of plant materials and the drug on the
nanoparticles. The XRD results showed that the synthesized particles have a crystalline structure and controlled size of about 14.5 nm.
Surface morphology of the iron oxide nanoparticles and drug loaded IONPs from SEM and TEM results show the cubic shaped particles
with less agglomeration. The silymarin loaded IONPs were tested against liver and lung cancer cells by MTT assay and fluorescence
microscopic analysis using various concentrations such as 10 µL, 25 µL and 50 µL. Results confirmed that the cell apoptosis was done by
nucleus fragmentations. Further, the quantitative cell apoptosis was done by flow cytometric method. The results confirmed that the
silymarin loaded IONPs disclosed excellent anticancer activity against lung cancer cells with 30.47% of post apoptotic cells over liver
cancer cells with 30.27% post apoptosis.

Item Type: Article
Uncontrolled Keywords: Iron oxide nanoparticles, Silymarin, Fluorescence, Flow cytometric, Cytotoxic analysis, A549 and HepG2 cancer cells
Divisions: PSG College of Arts and Science > Department of Carnatic Music
Depositing User: Mr Team Mosys
Date Deposited: 26 Sep 2022 06:21
Last Modified: 26 Sep 2022 06:21
URI: http://ir.psgcas.ac.in/id/eprint/1543

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