Shanmuga Priya, Ramasamy and Anitha, Rajendran and Priya, S and Muthukrishnan, Pallikondaperumal (2022) Broad-Spectrum Antimicrobial, Antioxidant, and Anticancer Studies of Leaf Extract of Simarouba glauca DC In Vitro. Antibiotics, 11 (59). pp. 1-17.

[thumbnail of 23.pdf] Text
23.pdf - Published Version
Download (4MB)

Abstract

The current study aimed to screen the preliminary phytochemicals in the leaf extract of
the medicinal plant Simarouba glauca and to analyze its potential antimicrobial, antioxidant and
anticancer properties. The phytochemical profile of the methanol extract was analyzed, and bioactive
compounds were identified using chromatography, FTIR and GCMS. Antimicrobial activity and
Minimum Inhibitory Concentration (MIC) were determined against 14 bacterial and 6 fungal strains.
Moreover, the synergistic effect of a plant extract with commercially available antibiotics was also
evaluated using the checkerboard method. The ethanolic and methanolic extracts showed exclusive
activity against S. aureus and profound activity against E. coli and S. marcescens. Upon comparing
breakpoints, methanolic extract demonstrated higher antimicrobial activity with a MIC value of
3.2 mg/mL against the test pathogens. Furthermore, the extracts demonstrated potential antioxidant
activity; methanol extract had higher antioxidant potential compared to the ethanol extract. The major
proactive bioactive compound with maximum antioxidant capacity was observed to be terpenoids.
The methanol extract of S. glauca showed significant cytotoxicity against the MCF-7 breast cancer cell
line with an IC50 value of 16.12 µg/mL. The overall results of our work provide significant evidence
for the usage of methanolic extract of S. glauca as an efficient ethnomedicinal agent and a potential
candidate for relieving many human ailments

Item Type: Article
Uncontrolled Keywords: Simarouba glauca; terpenoids; antimicrobial; antioxidants; anticancer; MCF-7 cell line
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 26 Sep 2022 09:08
Last Modified: 26 Sep 2022 09:08
URI: http://ir.psgcas.ac.in/id/eprint/1547

Actions (login required)

View Item
View Item
Altmetric