Sai Sugitha, Sasidharan and Renugadevi, Thiruppath Preferential Cytotoxic Effect of Vaccinium sect. Cyanococcus Fruit Extract in Human Lung Cancer Cells Related to Normal cells. Current Trends in Biotechnology and Pharmacy, 16 (3). pp. 396-406. ISSN 0973-8916

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Abstract

The study aimed to assess the
preferential cytotoxicity of the Vaccinium sect.
Cyanococcus fruit extract (VCFE) in human
lung cancer cells (A549) related to human
normal/healthy kidney cells (HEK-293).
VCFE was extracted using ethanol following
the cold maceration technique. A qualitative
phytochemical profile showed that VCFE was
rich in phenolics and flavonoids. A quantitative
phytochemical profile showed that one g of
VCFE was found to contain 26.31 ± 1.09 mg
gallic acid equivalent, GAE, 37.10 ± 1.82 mg
quercetin equivalent, and 6.27 ± 0.39 mg
tannic acid equivalent of total phenolics, total
flavonoids, and total tannins, respectively. The
EC50 value (effective concentration required to
inhibit 50% of free radicals) of VCFE in ABTS
free radical, DPPH free radical, and FRAP
antioxidant assays was determined as 372.81 ±
20.58, 414.63 ± 33.08, and 504.58 ± 27.76 µg/
mL, respectively. The IC50 value (concentration
required to inhibit 50% of cell viability) of VCFE
in human lung cancer cells (A549) and human
normal kidney cells (HEK-293) was determined
as 262.41 ± 23.03 and 608.05 ± 37.92 µg/mL,
respectively. The findings revealed that the
concentration of VCFE needed to inhibit the
growth of A549 cancer cells was substantially
lower than that needed to inhibit the growth of
HEK-293 normal cells, demonstrating VCFE’s selective cytotoxicity for cancer cells. The study
concluded that VCFE might be effective in
developing specific cancer-fighting medications.

Item Type: Article
Uncontrolled Keywords: Vaccinium sect. Cyanococcus, antioxidant activity, cytotoxicity, anticancer activity, A549 cells, HEK-293 cells.
Divisions: PSG College of Arts and Science > Department of Zoology
Depositing User: Mr Team Mosys
Date Deposited: 06 Oct 2022 06:11
Last Modified: 06 Oct 2022 06:11
URI: http://ir.psgcas.ac.in/id/eprint/1574

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