Jeyaraj, Senthil Kumar and Vishnu Priya, Kalimuthu (2022) SCREENING AND OPTIMIZATION OF POLYHYDROXY ALKANOATE (PHA) PRODUCTION FROM ISOLATED MICROBIAL STRAINS IN COIMBATORE, TAMILNADU, INDIA. New Materials, Compounds and Applications, 6 (2). pp. 148-161.

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Abstract

. Biodegradable polymers were produced instead of polythene bags by an array of microbes and
plants with potential impact to the consumers against environmental pollution. The samples were
collected from municipal garbage dumping yards in five localities of the study area. About 56 strains
were isolated from collected samples through serial dilution and screened for polyhydroxyalkanoate
(PHA) accumulation by sudan black B, nile blue A staining, and tributyrine hydrolysis tests. The PHA
accumulating isolates as M12a and M16a strains were identified, characterized through morphological,
cultural, biochemical, and molecular level characterization using 16S rRNA sequencing with the aid of
Basic Local Alignment Search Tool (BLAST) to conclude their identity of strains up to species level. The
PHA extracted was further confirmed by FTIR spectroscopy to detect the presence of functional groups,
with a peak at 1516 cm-1
and 1637 cm-1
corresponding to (N-O) and (C=O) groups indicating the presence
of PHA and bioactive compounds were further processed for Gas chromatography (GCMS) analysis. The
bacterial growth conditions were optimized for physical parameters, including temperature, pH, NaCl
concentration, incubation time. The effect of inexpensive carbon sources of rice, corn, potato, and tapioca
skin waste decreased production costs. The results obtained for the highest PHA accumulation were
observed with rice starch as 58.51% and 58.33%, in M16a and M12a, respectively.

Item Type: Article
Uncontrolled Keywords: Bioplastics, Polyhydroxyalkanoate, Bacillus licheniformis, Fourier Transform Infrared spectroscopy, GCMS, Nile blue A, Sudan black B, Optimization of pH, Temperature, Incubation time and NaCl concentration.
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 20 Oct 2022 04:22
Last Modified: 20 Oct 2022 04:22
URI: http://ir.psgcas.ac.in/id/eprint/1594

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