Boopathi, Balasubramaniam and Rajaselvi Darjily, Dharmaraj (2019) Exploration of the optimized parameters for bioactive prodigiosin mass production and its 2 biomedical applications in vitro as well as in silico. Biocatalysis and Agricultural Biotechnology, 22: 101385. ISSN 1878-8181

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Abstract

Prodigiosin from Serratia marcescens is considered as an important secondary metabolite for
various significant applications. This study explores the optimized conditions for the maximal
prodigiosin production, characterization of prodigiosin, biomedical applications against
therapeutically important diseases. Prodigiosin was extracted, purified using different solventsand the purified prodigiosin was characterized using FT-IR,
UV-Vis Spectrometry and GC-MS
analysis. The maximal production of prodigiosin was identified using different optimization
methods. UV-Vis spectrophotometry showed the absorbance peak at 530 nm and the FT-IR result revealed prodigiosin contains most of the functional groups that are corresponding to theliterature reports and also GC-MS analysis validated the presence of prodigiosin by showing the peak at 323 m/z. Antibacterial activity showed higher activity against Staphylococcus sp. than Gram negative bacteria and showed a very effective activity against Candida sp. than othertested fungi. The larvicidal activity of prodigiosin against Aedes aegypti clearly indicated that 32 % and 76 % mortality rate was seen at 24 and 48 h respectively. In silico analysis of prodigiosin
against Aβ peptide 1-42, a well-known breast cancer protein RAF-1 and two viral proteins E1A
and TMV-CP revealed that it has a strong interaction with less binding energy. Altogether, it is
strongly endorsed that the prodigiosin pigment could pave a way to treat various bacterial
infections and disorders in the forthcoming existences.

Item Type: Article
Uncontrolled Keywords: Serratia marcescens, Aedes aegypti, FT-IR, GC-MS, c-RAF and Aβ
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 21 Oct 2022 04:07
Last Modified: 21 Oct 2022 04:07
URI: http://ir.psgcas.ac.in/id/eprint/1616

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