Vishnu Priya, Kalimuthu and Senthil Kumar, Jeyaraj (2022) SCREENING AND OPTIMIZATION OF POLYHYDROXY ALKANOATE (PHA) PRODUCTION FROM ISOLATED MICROBIAL STRAINS IN COIMBATORE, TAMILNADU, INDIA. New Materials, Compounds and Applications, 6 (2). pp. 148-161.

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Abstract

Biodegradable polymers were produced instead of polythene bags by an array of microbes and plants with potential impact to the consumers against environmental pollution. The samples were collected from municipal garbage dumping yards in five localities of the study area. About 56 strains were isolated from collected samples through serial dilution and screened for polyhydroxyalkanoate (PHA) accumulation by sudan black B, nile blue A staining, and tributyrine hydrolysis tests. The PHA accumulating isolates as M12a and M16a strains were identified, characterized through morphological, cultural, biochemical, and molecular level characterization using 16S rRNA sequencing with the aid of Basic Local Alignment Search Tool (BLAST) to conclude their identity of strains up to species level. The PHA extracted was further confirmed by FTIR spectroscopy to detect the presence of functional groups, with a peak at 1516 cm-1 and 1637 cm-1 corresponding to (N-O) and (C=O) groups indicating the presence of PHA and bioactive compounds were further processed for Gas chromatography (GCMS) analysis. The bacterial growth conditions were optimized for physical parameters, including temperature, pH, NaCl concentration, incubation time. The effect of inexpensive carbon sources of rice, corn, potato, and tapioca skin waste decreased production costs. The results obtained for the highest PHA accumulation were observed with rice starch as 58.51% and 58.33%, in M16a and M12a, respectively.

Item Type: Article
Uncontrolled Keywords: Bioplastics, Polyhydroxyalkanoate, Bacillus licheniformis, Fourier Transform Infrared spectroscopy, GCMS, Nile blue A, Sudan black B, Optimization of pH, Temperature, Incubation time and NaCl concentration.
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 02 Jan 2023 08:42
Last Modified: 02 Jan 2023 08:42
URI: http://ir.psgcas.ac.in/id/eprint/1673

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