Shobana, CS (2022) Molecular identification, phylogeny and antifungal susceptibilities of dematiaceous fungi isolated from human keratomycosis. Journal of Infection and Public Health, 16 (4). pp. 25-33. ISSN 1876-0341

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Abstract

Purpose: To investigate the dematiaceous fungal profile of patients with ocular mycoses attending a tertiary
eye care hospital in Coimbatore, India
Methods: The identification of dematiaceous fungus based on their morphology, their genotypes, and the
measurement of the minimum inhibitory concentrations (MICs) using microdilution method of routinely
used antifungal drugs were all compared.
Results: A total of 148 dematiaceous fungi were isolated during a study period of 27 months. Isolates were
confirmed as Curvularia spp. (n = 98), Exserohilum spp. (n = 32), Alternaria spp. (n = 14), Exophiala spp.
(n = 2), Cladosporium sp. (n = 1) and Aureobasidium sp. (n = 1). Out of 50 well grown isolates characterized
genotypically based on the amplification and sequencing of the ITS region of the ribosomal RNA gene cluster
and subsequent BLAST analysis, Curvularia lunata (n = 24), C. aeria (n = 1), C. spicifera (n = 8), C. hawaiiensis
(n = 1), C. maydis (n = 2), C. papendorfii (n = 2), C. geniculata (n = 3), C. tetramera (n = 2) and Exs. rostratum
(n = 7) were identified. In vitro antifungal susceptibilities of the most tested dematiaceous isolates showed
that voriconazole had a MIC50 of 0.25 μg ml−1, while amphotericin B had a MIC50 of 0.25 μg ml−1 for
Curvularia spp. and Alternaria spp.
Conclusion: Voriconazole proved to be the most effective drug against the pigmented filamentous fungi,
followed by amphotericin B, itraconazole and econazole.

Item Type: Article
Uncontrolled Keywords: Keratomycosis Dematiaceous fungi Curvularia Molecular identification Antifungal susceptibilities
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 11 Mar 2023 07:12
Last Modified: 11 Mar 2023 07:12
URI: http://ir.psgcas.ac.in/id/eprint/1760

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