Hariharan, P and Sudha, A S and Agilandeswari, K (2021) Biotransformation of 3-cyanopyridine by utilizing free and immobilized whole cell nitrilase enzyme from Nocardia globerula NHB-2. Journal of RNA and Genomics formerly Journal of RNAi and Gene Silencing, 17 (S1). ISSN 2591-7781

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Abstract

Nitrile hydrolysing enzymes are potential biocatalyst for direct conversion of toxic nitriles into nontoxic and high value amides and acids. The microorganism Nocardia globerula NHB-2 was grown and
induced with three different short chain aliphatic nitriles and exhibited activity towards aromatic
nitriles observed. Propionitrile was found to be the optimal inducer for this microorganism at a
concentration of 0.3% v/v. Three factor Box Behnken design was used to optimize the reaction
parameters for the biotransformation of 3-cyanopyridine to nicotinic acid. The optimized conditions
include substrate concentration of 210 mM, resting cell concentration of 30 U/mg DCW and time of
conversion of 70 min. Then, immobilization of the resting cells of Nocardia globerula NHB-2 with agar
was performed and the nitrilase activity was compared with that of the free resting cells in terms of
thermal stability. Packed bed reaction system of the immobilized cells was utilised for the production
of nicotinic acid in a continuous operation wherein the substrate is recycled. The immobilized resting
cells were reused four times and the residual nitrilase activity observed was 40% up to the third
recycle.

Item Type: Article
Uncontrolled Keywords: Nitrilase, Response surface methodology, 3-cyanopyridine, Nicotinic acid, Immobilization, Agar
Divisions: PSG College of Arts and Science > Department of Biotechnology
Depositing User: Mr Team Mosys
Date Deposited: 01 Apr 2023 04:04
Last Modified: 01 Apr 2023 04:04
URI: http://ir.psgcas.ac.in/id/eprint/1837

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