Shobana, CS (2018) Expression and production optimization of the cationic antimicrobial peptide - indolicidin by the recombinant E. coli C41 (DE3) clones. Acta Biologica Szegediensis, 62 (1). pp. 61-66.

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Abstract

The cytoplasmic granules of bovine neutrophils naturally possess
indolicidin - a promising cationic antimicrobial peptide as it displays inherent inhibitory activities against a broad type of microbial pathogens. In this study, a shake flask
level production and expression optimizations of the indolicidin by the recombinant
Escherichia coli C41 (DE3) clones (transformed with pET21a(+) plasmid carrying indolicidin gene) were carried out under standard conditions, as to determine the conditions
required for maximal production. It was determined that a concentration of 1 mM of
IPTG was effective, the 2×YT with salts and LB media at pH 7.5 with 3-6 h of incubation
were required for maximal indolicidin expression

Item Type: Article
Uncontrolled Keywords: cationic antimicrobial peptide drug resistance indolicidin optimization
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 12 Apr 2023 04:07
Last Modified: 12 Apr 2023 04:08
URI: http://ir.psgcas.ac.in/id/eprint/1877

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