Hemalatha, D (2020) Transcriptional, Biochemical and Histological Alterations in Adult Zebrafish (Danio rerio) Exposed to Benzotriazole Ultraviolet Stabilizer - 328. Science of the Total Environment. pp. 1-37.

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Abstract

The occurrence of Benzotriazole Ultraviolet Stabilizer
-328 (BUV
-328) in different
environmental and biological matrices is of immediate environmental concern. In the present
study, we evaluated the toxicity of BUV
-328 in zebrafish liver tissues to understand the role
of oxidative damage in hepatotoxicity.
Adult zebrafish were exposed to 0.01, 0.1 and 1 mg/L of BUV
-328. At the end of 14, 28 and 42 days, liver tissues were examined for the responses
of antioxidant enzymes, gene expression and histopathological alterations. The results
indicated that superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx)
activities were elevated at concentrations of 0.1 and 1 mg/L on 14th and 28th day. Glutathione S-transferase (GST) activity and malondialdehyde (MDA) levels were elevated in all the
treated groups. The transcriptional levels of genes encoding sod, cat, gpx and gst enzymes
were increased at 14th day and then declined (except sod on 28th day). Moreover, transcription
of cyp1a, and hsp70 were up
-regulated throughout the study period. Histopathological lesions
such as hypertrophy, cellular and nuclear enlargement, cytoplasmic and nuclear degeneration,
necrosis with pyknotic nuclei, lipid and cytoplasmic vacuolization and nuclear displacement
to the periphery were found to be increased with the dose and exposure duration
. In brief, our
findings indicate that even a low dose of BUV
-328 is toxic to induce oxidative stress and
liver damage in zebrafish over a long period of exposure
.

Item Type: Article
Uncontrolled Keywords: Oxidative stress; Gene expression; Histopathology; Cytochrome P450 ; Heat shock proteins
Divisions: PSG College of Arts and Science > Department of Zoology
Depositing User: Mr Team Mosys
Date Deposited: 07 Jul 2023 07:00
Last Modified: 07 Jul 2023 07:00
URI: http://ir.psgcas.ac.in/id/eprint/1965

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