Deenathayalan Uvarajan and Brindha Durairaj (2022) Evaluation of Developmental Toxicity and Oxidative Stress Caused by Zinc Oxide Nanoparticles in Zebra fish Embryos/ Larvae. Evaluation of Developmental Toxicity and Oxidative Stress Caused by Zinc Oxide Nanoparticles in Zebra fish Embryos/ Larvae. ISSN 1559-0291

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Abstract

Zinc oxide nanoparticles (ZnO NPs) are used in various fields, including biological ones. ZnO NPs are
eventually disposed of in the environment where they may affect natural systems, and there is no
international law to regulate their manufacture, usage, and disposal. Hence, this present study is carried
out to synthesize a more non-toxic and bioactive ZnO NPs from the marine algae Sargassum polycystum.
The ZnO NPs were biologically produced using the marine algae Sargassum polycystum. The Dynamic
light scattering result describes that synthesized particles average size about 100nm in diameter
Transmission electron microscopy (TEM) analysis demonstrated the rod like morphology of ZnO NPs.
Fourier transmission infrared spectroscopy (FT-IR) results revealed the presence of functional groups in
ZnO NPs. The selected area electron diffraction (SAED) results strongly suggested the ZnO NPs
crystallinity. ZnO NPs surface morphology and compositions were identified by scanning electron
microscopy (SEM- EDX) values. To analyse the toxicity of synthesized nanoparticles zebra fish larvae
were used, which involved subjecting embryos to various ZnO NPs concentrations at 1 hpf and analysing
the results at 96 hpf. The 60 and 80 ppm sub-lethal doses were chosen for further studies based on the
LC50 (82.23 ppm). In the ZnO NPs treated groups, a significant slowdown in pulse rate and a delay in
hatching were seen, both of which impacted the embryonic processes. A teratogenic study revealed a
dose-dependent increase in the incidence of developmental deformities in the treated groups. Along with
increased oxidants and a corresponding reduction in antioxidant enzymes, Na+ K+-ATPase and AChE
activity changes were seen in ZnO NPs treated zebra fish larvae groups. The apoptosis process was
increased in ZnO NPs treated groups revealed by acridine orange staining. These results indicate that the
green synthesis process cannot mitigate the oxidative stress induced by ZnO NPs on oxidative signalling.

Item Type: Article
Uncontrolled Keywords: Zinc oxide nanoparticles, zinc accumulation, Teratogenicity, Nanotoxicity, zebrafish embryotoxicity test (ZET)
Divisions: PSG College of Arts and Science > Department of Chemistry
Depositing User: Mr Team Mosys
Date Deposited: 10 Sep 2024 09:50
Last Modified: 10 Sep 2024 09:50
URI: https://ir.psgcas.ac.in/id/eprint/2247

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