Vivekanandan, K.E (2025) Exploring molecular mechanisms of drug resistance in bacteria and progressions in CRISPR/Cas9-based genome expurgation solutions. Exploring molecular mechanisms of drug resistance in bacteria and progressions in CRISPR/Cas9-based genome expurgation solutions, 12 (2). pp. 1-16. ISSN 2699-9404

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Official URL: Elsevier B.V.

Abstract

Antibiotic resistance in bacteria is a critical global health challenge, driven by molecular me
chanisms such as genetic mutations, efflux pumps, enzymatic degradation of antibiotics, target
site modifications, and biofilm formation. Horizontal gene transfer (HGT) further accelerates the
spread of resistance genes across bacterial populations. These mechanisms contribute to the
emergence of multidrug-resistant (MDR) strains, rendering conventional antibiotics ineffective.
Recent advancements in CRISPR/Cas9-based genome editing offer innovative solutions to combat
drug resistance. CRISPR/Cas9 enables precise targeting of resistance genes, facilitating their
deletion or inactivation, and provides a potential method to eliminate resistance-carrying plas
mids. Furthermore, phage-delivered CRISPR systems show promise in selectively killing resistant
bacteria while leaving susceptible strains unaffected. Despite challenges such as efficient de
livery, off-target effects, and potential bacterial resistance to CRISPR itself, ongoing research and
technological innovations hold promise for using CRISPR-based antimicrobials to reverse bac
terial drug resistance and develop more effective therapies. These abstract highlights the mole
cular mechanisms underlying bacterial drug resistance and explores how CRISPR/Cas9 tech
nology could revolutionize treatment strategies against resistant pathogens

Item Type: Article
Uncontrolled Keywords: Keywords: Molecular mechanisms Drug resistance Bacteria CRISPR/Cas9 Genome editing Advancements
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 29 Sep 2025 09:30
Last Modified: 29 Sep 2025 09:30
URI: https://ir.psgcas.ac.in/id/eprint/2448

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