Vivekanandan, K.E (2025) Comprehensive investigation of antibacterial, cytotoxic, and antioxidant activities of Ipomoea staphylina flower extract with isolation and characterization of Beta-sitosterol. Comprehensive investigation of antibacterial, cytotoxic, and antioxidant activities of Ipomoea staphylina flower extract with isolation and characterization of Beta-sitosterol, 1336. pp. 1-9. ISSN 0022-2860

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Abstract

This study aims to bridge the gap by scientifically investigating the ethnopharmacological evidence surrounding
the I. staphylina plant. In this research we evaluated the antibacterial and cytotoxic abilities of I. staphylina flower
extracts. The extract with the highest antioxidant activity underwent cytotoxicity testing against HCT-116 cells.
Further analysis confirmed the presence of Beta-sitosterol through HPTLC, followed by its isolation via column
chromatography. Antibacterial tests showed significant activity against various gram-positive and gram-negative
bacteria, with notable resistance against S. aureus, P. mirabilis, and P. aeruginosa. The IPFPE extract exhibited the
highest inhibition against S. aureus (16 mm) and P. mirabilis (15 mm), while ethanolic extract showed potent
activity against S. aureus and P. aeruginosa (16 mm, 15 mm). The anticancer study, using ethanolic and pet-ether
extracts on HCT-116 colon cancer cells and assessed via the MTT assay, revealed significant reductions in cell
viability due to apoptosis induced by secondary metabolites (Beta-sitosterol) and antioxidants. A significant
reduction in cell viability was observed, with IC₅₀ values of 51.44 µM (IPFE) and 52.74 µM (IPFPE), a 50 %
growth inhibition was observed, highlighting vigorous pro-apoptotic activity. These results underscore the po
tential of I. staphylina flower extracts as promising antibacterial and chemotherapeutic agents, warranting further
research to validate their efficacy.

Item Type: Article
Uncontrolled Keywords: Cytotoxic study Plant extraction Antibacterial activity Colon cancer Beta-sitosterol Antioxidant
Divisions: PSG College of Arts and Science > Department of Microbiology
Depositing User: Mr Team Mosys
Date Deposited: 29 Sep 2025 10:00
Last Modified: 29 Sep 2025 10:00
URI: https://ir.psgcas.ac.in/id/eprint/2449

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