Aiswarya Gangadheran Nambiyar and Brindha Durairaj (2024) Characterisation and Toxicity Evaluation of Synthesized Phloridzin Chitosan Nanoparticles in in vivo Model. Characterisation and Toxicity Evaluation of Synthesized Phloridzin Chitosan Nanoparticles in in vivo Model, 59 (1). S102-S113.
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Characterisation and Toxicity Evaluation of Synthesized Phloridzin Chitosan Nanoparticles in in vivo Model.pdf - Published Version
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Abstract
Phloridzin, a plant compound is of interest in the field of research due to its
antioxidant, anti-inflammatory, anticancer and antidiabetic activity. The current study's objectives
were to create, characterize and evaluate the toxicity of synthesized phloridzin chitosan
nanoparticles in Sprague Dawley rats. Materials and Methods: The chitosan nanoparticle was
synthesized by ionic gelation method. The synthesized nanoparticles were characterized using
different techniques like Dynamic Light Scattering, Fourier Transform Infrared Spectroscopy, X-ray
Diffraction, Fourier Emission Scanning Electron Microscopy and %Drug Entrapment Efficiency.
The toxicity and cellular uptake of phloridzin chitosan nanoparticles were further evaluated
by in vitro and in vivo methods. MTT and cellular uptake assay were carried out in SH-SY5Y
cells. Following in in vivo Lorke method and the Organization for Economic Co-operation and
Development 407, the sub-chronic toxicity of phloridzin chitosan nanoparticles was investigated.
Results: Chitosan encapsulated phloridzin nanoparticles showed an average particle size of 246.6
d.nm in Dynamic Light Scattering analysis with a polydispersity index of 0.526 and zeta potential
of 30.9mv. Fourier Transform Infrared Spectroscopy and X-ray Diffraction analysis confirmed the
presence and stability of phloridzin-encapsulated chitosan nanoparticles. In Fourier Emission
Scanning Electron Microscopy analysis, the dehydrated sample size was found to be 186 nm.
Drug entrapment efficiency was found to be 50.38%. Cell viability range was above 60% after 48
hr incubation. Cellular uptake of nanoparticles was confirmed. The data obtained from the Lorke
method indicated that LD50
exceeded 5000 mg/kg. No significant alterations were observed in
hematological, biochemical and histopathological studies. Conclusion: The findings suggest
that the Phloridzin nanoparticles encased in chitosan did not significantly produce toxicity in
both in vitro and in vivo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Phloridzin, Chitosan nanoparticle, Toxicity, Liver, Kidney, Brain |
| Divisions: | PSG College of Arts and Science > Department of Biotechnology |
| Depositing User: | Dr. B Sivakumar |
| Date Deposited: | 27 Oct 2025 09:17 |
| Last Modified: | 27 Oct 2025 09:17 |
| URI: | https://ir.psgcas.ac.in/id/eprint/2471 |
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