Srilatha, M and Lavanya, K (2021) In vitro antioxidative and cytotoxic effect of protein fraction from divaricata leaves against DLA cells. In vitro antioxidative and cytotoxic effect of protein fraction from divaricata leaves against DLA cells, 7 (1). pp. 17-21. ISSN 2455-2674

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Abstract

t
Background: The rising burden of cancer worldwide calls for an alternative treatment solution. Herbal medicine
provides a very feasible alternative to western medicine against cancer.
Objectives:
vitro
antioxidative potential and cytotoxicity of
Tabernaemontana divaricata
The present study assessed the
in
leaves protein fraction Materials and
.
Methods: The antioxidant activity of protein fraction of
T. divaricata
was performed by several antioxidative assays
including 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ABTS, superoxide radical (SO)
scavenging assay and Hydrogen peroxide (H O ) with standard protocol, followed by Cytotoxicity test with tryphan
2 2
blue assay.
Results:
From the results,
T.divaricata
has been found to have the significant antioxidant activity in a dose
dependent manner and IC value was 59.1μg/ml for DPPH, 48µg/ml for ABTS, 60 µg/ml for SO and 38.5µg/ml for
50
2 2
HO. Further, the cytotoxicity analysis was determined against Dalton Lymphoma Ascites (DLA) cell line and the IC
value was found to be 62μg/ml for protein fraction of
T.divaricata.
Hence, the current study attests that
50
Conclusion:
divaricata is a fine source of natural antioxidants with anticancer agents and can be used in pharmaceutical preparations
for the treatment of diseases induced by oxidative stress.

Item Type: Article
Uncontrolled Keywords: T. divaricata, antioxidants, dalton lymphoma ascites, trypan blue assay
Divisions: PSG College of Arts and Science > Department of Biochemistry
PSG College of Arts and Science > Department of Biotechnology
Depositing User: Dr. B Sivakumar
Date Deposited: 26 Feb 2026 07:01
Last Modified: 26 Feb 2026 07:01
URI: https://ir.psgcas.ac.in/id/eprint/2660

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