Kunnathur Murugesan Sakthivel (2024) Biosynthesis of chitosan encapsulated silver- nanoparticles using Probiotic-Lactobacillus plantarum strain and it’s in vitro anticancer assessment on HeLa cells. Biosynthesis of chitosan encapsulated silver- nanoparticles using Probiotic-Lactobacillus plantarum strain and it’s in vitro anticancer assessment on HeLa cells: 100117. pp. 1-11. ISSN 2590-0978
Full text not available from this repository.Abstract
Cervical cancer remains the deadliest cancer among women worldwide. Investigating the molecular mechanisms
of tumor progression by targeting signaling pathways can provide insights into novel therapeutic strategies to
overcome the limitations of conventional treatments. Green synthesis of nanoparticles addresses conventional
treatment drawbacks like chemotherapy and radiation. This study aims to green synthesize, characterize, and
evaluate chitosan-encapsulated silver nanoparticles (AgNPs) using Lactobacillus plantarum probiotics against
cervical cancer HeLa cells, targeting the EMT mechanism. The green synthesized chitosan encapsulated Silver-
Nanoparticles using probiotic -Lactobacillus plantarum (CS-LP-AgNPs) were characterized using UV–vis spec-
troscopy, which showed a peak at 420 nm confirming the reduction of AgNPs. Zeta and DLS analysis revealed the
particle surface charge and stability. TEM analysis demonstrated that CS-LP-AgNPs are spherically shaped, with a
size of approximately 15.3 nm. XRD patterns confirmed the crystalline nature of CS-LP-AgNPs. FTIR spectroscopy
confirmed that CS-LP-AgNPs were functionalized with biomolecules. DAPI and double staining were employed to
examine characteristic nuclear and morphological changes during apoptosis. Gene expression profiles of EMT in
HeLa cell lines were performed to evaluate the anticancer potency of CS-LP-AgNPs. MTT assay demonstrated
cytotoxic activity, whereas DAPI/(AO/EB) double staining images showed the induced apoptosis in HeLa cells by
CS-LP-AgNPs treatment. CS-LP-AgNPs treated HeLa cells showed decreased SNAIL/EMT via enhancing apoptotic
cascade mechanism. Green synthesized CS-LP-AgNPs may be considered an effective anti-cancer drug delivery
system for the treatment of CC in the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Chitosan encapsulation Silver nanoparticle Cervical cancer Lactobacillus plantarum EMT pathway |
| Divisions: | PSG College of Arts and Science > Department of Biochemistry |
| Depositing User: | Dr. B Sivakumar |
| Date Deposited: | 28 Feb 2026 09:16 |
| Last Modified: | 28 Feb 2026 09:16 |
| URI: | https://ir.psgcas.ac.in/id/eprint/2671 |
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