Kunnathur Murugesan Sakthivel and Rajan Radha Rasmi and Kavitha Thangavelu (2025) Multifaceted Evaluation of Nano Encapsulated Polymeric Scopoletin Against HT‐29 Colon Cancer Cells. Multifaceted Evaluation of Nano Encapsulated Polymeric Scopoletin Against HT‐29 Colon Cancer Cells. pp. 127-144.

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Abstract

In the sphere of medical research, nanoencapsulated polymeric drugs in biological circumstances are an intriguing issue for
therapeutical diagnosis. The nanoprecipitation approach was employed in thisstudy to synthesisthe nanoencapsulated polymeric
scopoletin (NEP‐Sc) and tested its effect against the HT‐29 cell line (human colorectal adenocarcinoma cells). Further, the syn�thesized NEP‐Sc was then characterized by ultra‐visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR),
X‐ray diffraction (XRD), field emission scanning electron microscopy (FE‐SEM) with energy dispersiveX‐ray analysis(EDAX), and
dynamic light scattering (DLS) analysis. All the analysis confirmed the effective synthesis of NEP‐Sc. The synthesized NEP‐Sc was
validated as anticancer drug through the conventional MTT assay, neutral red uptake assay, apoptosis assay using AO/EB and
lactate dehydrogenase assay and trypan blue assay against HT‐29 cells. The docking studies ofscopoletin with multiple genes were
also carried out using the PyRx—virtual screening tool. The findings indicated that increase in concentration of NEP‐Sc exhibited
dose dependent action of cell death against the HT‐29 colon cancer cells. To summarize, the observed results of anticancer and
computational modeling studies underscores the therapeutic potential of NEP‐Sc in treating colon cancer.

Item Type: Article
Uncontrolled Keywords: cancer | colorectal adenocarcinoma | docking | HT‐29 | nanoencapsulation | scopoletin
Divisions: PSG College of Arts and Science > Department of Biotechnology
PSG College of Arts and Science > Department of Biochemistry
Depositing User: Dr. B Sivakumar
Date Deposited: 05 Mar 2026 06:22
Last Modified: 05 Mar 2026 06:22
URI: https://ir.psgcas.ac.in/id/eprint/2679

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