Sakthivel Kunnathur Murugesan (2024) Differential expression of nickel nanoparticles of Lactobacillus plantarum on VDR/LncRNA EIF3J-DT in Colorectal cancer. Differential expression of nickel nanoparticles of Lactobacillus plantarum on VDR/LncRNA EIF3J-DT in Colorectal cancer, 12: 100032. ISSN 2590-1249
![[thumbnail of 51.Differential expression of nickel nanoparticles of Lactobacillus plantarum on VDR_LncRNA EIF3J-DT in Colorectal cancer.pdf]](https://ir.psgcas.ac.in/style/images/fileicons/text.png)
Text
51.Differential expression of nickel nanoparticles of Lactobacillus plantarum on VDR_LncRNA EIF3J-DT in Colorectal cancer.pdf - Published Version
Download (8MB)
Abstract
Colorectal cancer (CRC) is the third most prevalent cancer worldwide. Vitamin D receptor (VDR) gene mutations
and Vitamin D deficiency contribute to CRC development and progression. Certain long non-coding RNAs
(lncRNAs) directly inhibit VDR gene transcription, leading to VDR mutation. Thus, targeting oncogenic lncRNAs
and VDR expression is a promising strategy for effective cancer treatment. Here, we green-synthesized Lacto�bacillus plantarum loaded nickel oxide nanoparticles (LpNiONPs) to assess their anticancer potential in CRC by
targeting long non-coding RNA EIF3J- divergent transcript (lncRNA EIF3J-DT) and VDR. The potent bioactive
component present in L. plantarum was identified via gas chromatography-mass spectrometry (GC–MS) analysis,
and its interaction with VDR, as well as the functional interaction with lncRNA EIF3J-DT, were evaluated using
the PyRx program and RPISeq-software, respectively. The LpNiONPs were characterized using UV–Vis spec�troscopy, Zeta Potential, dynamic light scattering (DLS), fourier transform infrared spectroscopy (FTIR), scanning
electron microscopy (SEM), energy dispersive X-ray (EDX) and X-ray diffraction (XRD) techniques. The anti�cancer potential of LpNiONPs against HT-29 cells was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphe�nyltetrazolium bromide (MTT) assay, scratch assay, 4′,6-diamidino-2-phenylindole (DAPI)/ acridine orange�ethidium bromide (AO-EtBr) staining experiments, and reverse transcriptase-PCR to evaluate the expression of
lncRNA EIF3J-DT/VDR and apoptotic-related genes. The potent bioactive compound Pyrrolo (1,2-a) pyrazine�1,4-dione in L. plantarum strongly interacts with VDR, highlighting its drug design potential. The formation of
LpNiONPs was confirmed via UV–Vis spectroscopy with an absorption peak at 394 nm. LpNiONPs were posi�tively charged, monodispersed, and stable square-shaped nanoparticles. LpNiONPs show dose-dependent cyto�toxicity and induced apoptosis, confirmed by staining images in HT-29 cells. Moreover, LpNiONPs
downregulated lncRNA EIF3J-DT, CYP24-A1 and BCL2 genes while upregulating VDR, cas-3, cas-9 and BAX in
HT-29 cells. These findings suggest that LpNiONPs exhibit anticancer activity by promoting VDR-associated
apoptosis by inhibiting lncRNA EIF3J-DT in CRC cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Colorectal Cancer lncRNA EIF3J-DT VDR Lactobacillus plantarum Nickel oxide nanoparticl |
| Divisions: | PSG College of Arts and Science > Department of Biochemistry |
| Depositing User: | Dr. B Sivakumar |
| Date Deposited: | 25 Mar 2026 06:52 |
| Last Modified: | 25 Mar 2026 06:52 |
| URI: | https://ir.psgcas.ac.in/id/eprint/2732 |
Actions (login required)
- View Item