Rajan Thinagaran (2025) An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple�negative breast cancer cells with Psidium guajava peel�derived chitosan nanopartic. An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guajava peel-derived chitosan nanoparticles, 53 (1). pp. 43-55.

[thumbnail of An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guaj.pdf] Text
An integrated in vitro and in silico approach to assess targeted cytotoxicity against MDA-MB-231 triple-negative breast cancer cells with Psidium guaj.pdf - Published Version
Download (6MB)

Abstract

Triple-negative breast cancer (TNBC) is a significant global health issue, with high mortality rates. The
chemotherapeutic drugs currently used for TNBC have significant side effects, impacting both normal and
cancer cells. In this study, we investigated a potential use of fruit peel extract of Psidium guajava (PGP)
encapsulated with chitosan nanoparticles (CSNPs) to combat TNBC. The synthesized PGP-CSNPs were
characterized using UV–vis spectroscopy, Fourier transform infra-red (FTIR) spectroscopy, TEM and GC–MS.
The maximum loading capacity and encapsulation efficacy of PGP-CSNPs were found to be 72.5 ± 0.49%
and 92.9 ± 0.10%, respectively. Furthermore, in vitro cytotoxicity was assessed, and the IC50 value for
PGP-CSNPs was 50.13 µg/mL. It was observed that PGP-CSNPs could induce apoptosis in MDA-MB-231
cells in dose-dependent manner. Furthermore, molecular docking was performed for bioactive compounds
retrieved from PGP-CSNPs against human tumour suppressor proteins Bcl2, and results showed that the
PGP-CSNPs had lower binding energy than cisplatin. This suggests that, the synthesized PGP-CSNPs have
the potential to serve as a therapeutic agent for tackling TNBC. However, to validate its efficacy in human
therapy, furthermore pre-clinical and clinical procedures should be examined, as this is an ongoing and
significant step towards developing an effective and safe anticancer drug.

Item Type: Article
Uncontrolled Keywords: TNBC; Psidium guajava peel (PGP); chitosan nanoparticles; FTIR; TEM; molecular docking
Divisions: PSG College of Arts and Science > Department of Biochemistry
Depositing User: Dr. B Sivakumar
Date Deposited: 26 Mar 2026 09:13
Last Modified: 27 Mar 2026 08:51
URI: https://ir.psgcas.ac.in/id/eprint/2745

Actions (login required)

View Item
View Item