Suseela, V (2015) CYTOTOXIC EFFECT OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING Indigofera longeracemosa ON SKIN CANCER SK MEL-28 CELL LINES. International Journal of Preclinical & Pharmaceutical Research, 6 (3). pp. 118-125. ISSN 2249-7552

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Abstract

The present study is focused on the biosynthesis of stable silver nanoparticles (AgNPs) from Indigofera longeracemosa and its anticancer effects in human skin cancer skin cell line SK-MEL-28 was investigated. The AgNPs synthesis was determined by UV- visible spectrum and it was further characterized by FT-IR, Scanning Electron Microscopy
(SEM), and X-Ray Diffraction (XRD) analysis. The cytotoxicity of the nanoparticles was determined against SK-MEL-28 cell line at different concentrations by MTT assay. Cell morphology and DNA fragmentation were also analyzed using AgNps of Indigofera longeracemosa. Also since tumorigenesis is thought to result from a series of progressive gene alterations, including activation of oncogenes and inactivation of tumour suppressor genes, expression of two such genes, P53 and Bcl-2 that are
believed to play a crucial role in tumourigenesis was determined. UV visible spectrum indicated an absorption peak at 436 nm which reflects its specific Surface Plasmon Resonance (SPR). Biosynthesized AgNPs were predominantly spherical and appeared in the size ranging from 30nm to 110nm as observed through SEM. Cytotoxicity of biosynthesized AgNPs against in vitro Human skin cancer cell line SK MEL-28 showed a dose-response activity with an IC50 value of 48µg/ml. DNAN fragmentation assay confirmed induction of apoptosis in SK-MEL-28 cells treated with AgNps of Indigofera longeracemosa. Apoptotic cells were characterized by condensed and fragmented DNA. From RT-PCR analysis it was clear that, AgNPs of Indigofera longeracemosa decreased the expression of anti apoptotic gene Bcl-2 significantly and increased the expression of
tumour suppressor gene p53. Since the AgNPs of Indigofera longeracemosa observed to be most effective in cell growth
inhibition and induction of apoptosis in SK-MEL-28 cells, it can be considered as effective cytotoxic agent. Further toxicity studies of AgNPs of Indigofera longeracemosa on animal models might open a door for a range of anticancer agents.

Item Type: Article
Uncontrolled Keywords: Indigofera longeracemosa, SK MEL-28 cell line, Anticancer activity, Silver nano particles, XRD, SEM
Depositing User: Users 1 not found.
Date Deposited: 25 Jan 2022 05:59
Last Modified: 25 Jan 2022 05:59
URI: http://ir.psgcas.ac.in/id/eprint/276

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